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cd11b positive selection  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd11b positive selection
    Cd11b Positive Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+positive+selection/CD11b+MicroBeads%2C+human+and+mouse+-+lyophilized/pm42212624-36-8-17
    Average 96 stars, based on 83 article reviews
    cd11b positive selection - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway.
    Article Snippet: .. To obtain primary astrocytes cultures, mixed glial cells were isolated from cortices of murine pups, and after the cultures reached full confluence, the microglia were removed by Cd11b- positive selection (Miltenyi Biotec, Bergisch Gladbach, Germany); the remaining cells were passaged once and then replated for different assays. ..

    Article Title: Distinct tissue-specific roles for the disease-associated autophagy genes ATG16L2 and ATG16L1
    Article Snippet: Splenic B and T cells and bone marrow neutrophils were isolated by negative selection using magnetic cell sorting (130–090-862, 130–095-130, 130–097-658, Miltenyi Biotec, CA). .. Splenic macrophages were isolated by CD11b positive selection (130–049-601, Miltenyi Biotec, CA). ..

    Article Title: Androgen Signaling Restricts Glutaminolysis to Drive Sex-Specific Th17 Metabolism
    Article Snippet: Cells were resuspended in MACS buffer (PBS with 2% FBS and 2 mM EDTA) and counted using trypan blue with the TC20 Automated Cell Counter (BioRad). .. Cell suspensions were fractionated using magnetic selection with the following: CD11b positive selection (Miltenyi Biotec) using LD columns (Miltenyi Biotec) for depletion followed by CD4 positive selection using LS columns (Miltenyi Biotec) for isolation on a Miltenyi QuadroMACS Separator according to manufacturer’s instructions. .. Fractions were resuspended in 1 mL medium and used for cell counts using trypan blue, flow cytometry for fraction composition, and radioactivity measurement using the Hidex Automatic Gamma Counter.

    Selection:

    Article Title: Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway.
    Article Snippet: .. To obtain primary astrocytes cultures, mixed glial cells were isolated from cortices of murine pups, and after the cultures reached full confluence, the microglia were removed by Cd11b- positive selection (Miltenyi Biotec, Bergisch Gladbach, Germany); the remaining cells were passaged once and then replated for different assays. ..

    Article Title: Distinct tissue-specific roles for the disease-associated autophagy genes ATG16L2 and ATG16L1
    Article Snippet: Splenic B and T cells and bone marrow neutrophils were isolated by negative selection using magnetic cell sorting (130–090-862, 130–095-130, 130–097-658, Miltenyi Biotec, CA). .. Splenic macrophages were isolated by CD11b positive selection (130–049-601, Miltenyi Biotec, CA). ..

    Article Title: Androgen Signaling Restricts Glutaminolysis to Drive Sex-Specific Th17 Metabolism
    Article Snippet: Cells were resuspended in MACS buffer (PBS with 2% FBS and 2 mM EDTA) and counted using trypan blue with the TC20 Automated Cell Counter (BioRad). .. Cell suspensions were fractionated using magnetic selection with the following: CD11b positive selection (Miltenyi Biotec) using LD columns (Miltenyi Biotec) for depletion followed by CD4 positive selection using LS columns (Miltenyi Biotec) for isolation on a Miltenyi QuadroMACS Separator according to manufacturer’s instructions. .. Fractions were resuspended in 1 mL medium and used for cell counts using trypan blue, flow cytometry for fraction composition, and radioactivity measurement using the Hidex Automatic Gamma Counter.

    Article Title: Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway
    Article Snippet: To obtain primary astrocytes cultures, mixed glial cells were isolated from cortices of murine pups (postnatal day 1–3) and maintained in vented capped T75 or T175 flasks (Corning, NY, USA) in high glucose Dulbecco’s modified Eagle’s medium (Nacalai Tesque) supplemented with 10% fetal bovine serum (Nichirei Biosciences, Tokyo, Japan), 100 U/mL penicillin, 100 mg/mL streptomycin, and 0.5 mM L-glutamine (all purchased from Nacalai Tesque). .. The medium was changed every 3–4 d, and after the cultures reached full confluence, the microglia were removed either by shaking vigorously or by Cd11b-positive selection (Miltenyi Biotec, Bergisch Gladbach, Germany); the remaining cells were passaged once or twice then replated for different assays. .. The astrocytes were treated with DMSO, 5 μM SLF, 100 μM DEM, or 10 μM Δ15-PGJ2 for 1 h, then stimulated with the cytokine cocktail containing 3 ng/mL IL-1α (Peprotech, NJ, USA, 211-11A, 30 ng/mL TNF α (Peprotech, 315-01A), and 400 ng/mL C1q (Prospec, Rehovot, Israel, PRO-554) for 4 h for quantitative RT-PCR (qPCR), ChIP-seq, and nascent RNA-seq analyses and for 24 h for western blotting and immunocytochemistry. iPSC culture Human iPS cell line 201B7-Ff derived from a healthy individual was obtained from RIKEN BRC.

    Article Title: CCAR2 reduces the number of osteoclasts by controlling osteoclast apoptosis.
    Article Snippet: The cell suspension was then passed through a 70-μm mesh filter screen, red blood cells were removed using ACK lysis buffer (A1049201; Gibco), and the cells were placed in a culture dish. .. The following day, the supernatant was collected, and CD11b positive selection was performed using CD11b MicroBeads (130-097-142; Miltenyi Biotec, San Diego, USA). ..

    Article Title: Fluoxetine Potentiates Phagocytosis and Autophagy in Microglia
    Article Snippet: Briefly, the brains of wild-type C57Bl6/J mice (3 months old) were removed immediately after euthanasia and digested with digestion buffer (Miltenyi Biotec, 130-107-677) at 37°C for 40 min. After the removal of myelin debris, the cell suspension was filtered through a 70-μm cell strainer. .. Cell pellets were washed with phosphate-buffered saline (PBS) with 0.5% bovine serum albumin (BSA), followed by CD11b-positive selection (Miltenyi Biotec, 130-093-636) using a mini-MACS (Miltenyi Biotec, 130-042-201) column. ..

    Article Title: BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia
    Article Snippet: .. The remaining cell suspension was filtered through a 40 μm strainer followed by CD11b + positive selection using the mini-MACS (Miltenyi Biotec Cat#130-042-201) column. ..

    Article Title: BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.
    Article Snippet: .. The remaining cell suspension was filtered through a 40 μm strainer followed by CD11b+ positive selection using the mini-MACS (Miltenyi Biotec Cat#130-042201) column. ..

    Saline:

    Article Title: Fluoxetine Potentiates Phagocytosis and Autophagy in Microglia
    Article Snippet: Briefly, the brains of wild-type C57Bl6/J mice (3 months old) were removed immediately after euthanasia and digested with digestion buffer (Miltenyi Biotec, 130-107-677) at 37°C for 40 min. After the removal of myelin debris, the cell suspension was filtered through a 70-μm cell strainer. .. Cell pellets were washed with phosphate-buffered saline (PBS) with 0.5% bovine serum albumin (BSA), followed by CD11b-positive selection (Miltenyi Biotec, 130-093-636) using a mini-MACS (Miltenyi Biotec, 130-042-201) column. ..

    Suspension:

    Article Title: BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia
    Article Snippet: .. The remaining cell suspension was filtered through a 40 μm strainer followed by CD11b + positive selection using the mini-MACS (Miltenyi Biotec Cat#130-042-201) column. ..

    Article Title: BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.
    Article Snippet: .. The remaining cell suspension was filtered through a 40 μm strainer followed by CD11b+ positive selection using the mini-MACS (Miltenyi Biotec Cat#130-042201) column. ..



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